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ICSI – Intracytoplasmic Sperm Injection

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ICSI – Intracytoplasmic Sperm Injection

This technique has been the greatest boon to male factor infertility which does not respond to conventional management, i.e. men with low sperm count and motility. As with IVF, the oocytes are aspirated. On a special microscope with a micromanipulator, a single sperm is picked up in a fine microneedle and injected manually into the oocyte. Embryos are transferred after 48 to 72 hours.

For azoospermic men with an obstructive pathology (e.g.: Congenital absence of vas deferens) sperm can be aspirated directly from the epididymis (PESA) and used for ICSI. In addition, for azoospermic men with a non-obstructive pathology, sperm can be retrieved directly from the testis (TESE) and used for ICSI.

Indications for ICSI:

  • Sperm completely absent in semen (Azoospermia)
  • Sperm present in poor concentration
  • Poor Sperm motility
  • Poor Sperm Morphology
  • History of fertilization failure with conventional IVF
  • Sperm retrieved by surgical techniques (TESE/ PESA)
  • If pre-implantation genetic diagnosis (PGD) is being used to screen embryos for some specific genetic disorder
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